Methods Mol Biol. This very low efficiency, combined with safety concerns, presents a serious obstacle to the application of reproductive cloning. Future and Applications of Cloning Alan O Trounson. Reproductive cloning is a very inefficient technique and most cloned animal embryos cannot develop into healthy individuals. Therapeutic cloning, where the nucleus from a donor cell is transferred into an enucleated oocyte in order to extract pluripotent embryonic stem cells, offers a potentially limitless source of cells for tissue engineering applications. For instance, Dolly was the only clone to be born live out of a total of 277 cloned embryos. 2006;348:319-32. doi: … Cloning technology has made several steps forward in recent years, most notably in somatic cell cloning and in combining cloning with transgenic technologies. Show details Methods Mol Biol Actions. This chapter explores some of the immediate uses of cloning technology in medicine and agriculture, and focuses on the applications … The present chapter reviews recent advances that have occurred in therapeutic cloning … Application of Gene Cloning:-Gene cloning is applicable in many fields of science such as medicine and agriculture. Search in PubMed Search in NLM Catalog Add to Search . 2006. Medical Applications:-In medicine, bacteria play a vital role for the synthesis …

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